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KMID : 1134820200490101054
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 10 p.1054 ~ p.1060
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Effects of the Mixture of Eucommia ulmoides Oliver and Achyranthes japonica Nakai Extracts (KGC08EA) on Testosterone Synthesis in Late-Onset Hypogonadism (LOH) in vitro Models
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Kim Seok-Ho
Lee Jeong-Yoon
Kim So-Hyuk
Kim Jong-Han
Kwon Han-Ol
Lee Jeong-Seon
Jun Woo-Jin
Lee Yoo-Hyun
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Abstract
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Late-onset hypogonadism (LOH) is associated with a significant decrease in the testosterone levels resulting from the reduced ability to synthesize testosterone in the Leydig cells as aging progresses. This study measured the testosterone levels after treating H2O2-stressed TM3 cells in the presence of human chorionic gonadotropin (hCG) with Eucommia ulmoides Oliver extracts (eu), Achyranthes japonica Nakai extracts (aj), or amixture of eu and aj extracts in the proportion of KGC08EA (1:3, 1:1, 3:1). KGC08EA (3:1) at concentrations of 1 to 500 μg/mL produced a significant concentration-dependent increase (up to 34 times (P<0.001)) in testosterone levels compared to the control group. In addition, among the testosterone synthesis enzymes, 17β-HSD1 and 3β-HSD4 showed significant increases in mRNA expression while both 5α-reductase2 and aromatase, such as testosterone degradation enzyme, was not increased significantly (P<0.05). In conclusion, KGC08EA (3:1) might bea health functional food material that can improve LOH by up-regulating the testosterone synthesis enzymes and increasing the testosterone levels significantly.
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KEYWORD
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late-onset hypogonadism, Achyranthes japonica Nakai, Eucommia ulmoides Oliver, testosterone, TM3 cell
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